Maharashtra on Sunday reported 40,414 fresh coronavirus cases, which is the highest rise so far in a single day.
With 108 fresh fatalities due to Covid-19 infection, the overall toll in Maharashtra reached 54,181.
Mumbai also reported the highest number of new cases in a day on Sunday, by adding 6,933 infections, and taking the overall tally to 3,98,724.
With eight more deaths, Mumbai's fatality count rose to 11,653, the department said.
In Maharashtra, 17,874 patients were discharged during the day, taking the total count of recoveries to 23,32,453.