Juventus on Monday were given a 10-point deduction in Serie A after a revision of their initial 15-point penalty inflicted on the club over illicit transfer activity.
The new sanction, announced by the Italian Football Federation's (FIGC) appeals court, severely dents Juventus' hopes of qualifying for next season's Champions League, pushing them out of the top four with two matches remaining this season.
Juventus have competed in Europe's elite competition in each of the past 11 seasons dating back to 2012.
Juve are now five points behind fourth-placed AC Milan and host the losing Champions League semi-finalists in their penultimate match of the campaign this weekend.
The 'Old Lady' had a chance to move within two points of Milan when they took on Empoli shortly after the points deduction was announced on Monday.
But they slipped to a surprising 4-1 defeat to cap a miserable day for the two-time European champions.
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